Fig. 5
From: RIPK1 suppresses apoptosis mediated by TNF and caspase-3 in intervertebral discs
Long-term RIPK1 knockdown triggered apoptosis through the cleaved caspase 3 pathway, while downregulating NF-κB and MAPKs cascades in primary chondrocyte cells. a mRNA levels of TNF 15 days after shRIPK1 knockdown detected by qRT-PCR. b mRNA levels of TNFAIP3, CCL2, and IκBα 15 days after shRIPK1 knockdown and at the indicated time after TNF stimulation, detected by qRT-PCR. c Detection of p-IKKα/β, p-JNK, p-p38, and cleaved caspase 3 in the protein lysates using western blotting. Data indicate the mean values calculated from triplicate samples from multiple independent experiments (n ≥ 3) (± SD). *P < 0.05; **P < 0.01