Fig. 3From: RIPK1 suppresses apoptosis mediated by TNF and caspase-3 in intervertebral discsShort-term RIPK1 knockdown led to inflammation in primary chondrocyte cells. a Construction of shRIPK1 vectors. b mRNA levels of RIPK1 5 days after shRIPK1 knockdown detected by qRT-PCR. c Representative Western blot results of shRIPK1 knockdown after 5 days. d Inflammatory cytokines levels 5 days after shRIPK1 knockdown. Data indicate the mean values calculated from triplicate samples from multiple independent experiments (n ≥ 3) (± SD). Differences were between shRIPK1 groups and the shControl group. *P < 0.05; **P < 0.01; ***P < 0.001Back to article page