Fig. 5

HIF-1α protein expression: Semiquantitative assessment of HIF-1α in unpooled/individual samples by western blotting shows an increased amount of HIF-1α after I/R-injury. RIPC significantly increases the amount of HIF-1α after I/R injury, but does not change the HIF-1 α protein levels in sham operated animals. Images on top show representative bands from 3 out of 5 or 10 experiments. Columns represent the mean of 5–10 independent experiments. Bars denote SD.*P < 0.05; ***P < 0.001. a.u. arbitrary units