Fig. 9
Effect of MSC on endothelial barrier function. Monolayer transendothelial electrical resistance (TEER) of HPMVEC cells treated with control MSC media, 10% conditioned media (CM), 30% CM, or 50% CM, generated from manually expanded Pig 1, Pig 2, or Pig 3 cells (a), and their effect against thrombin challenge (b). The effect of CM generated from QE-2 Pig 1 and Pig 3 cells on HMVEC barrier resistance (c), and their effect against thrombin challenge (d). AUC box plots represent area under the curve quantitation of barrier resistance as mean ± STD. % Change in resistance boxplots represent a maximum decrease in barrier resistance as mean ± STD. These are calculated based on 4 wells/condition/donor/treatment (dose). *Indicates significant difference from control in a, c, while * indicates a significant difference from thrombin in b, d, as determined by one-way ANOVA (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001)