Fig. 7

Frequency of MSC markers is maintained on QE1, QE2 and manually expanded passage 3 cells. Cryopreserved MSCs from donor Pigs 1, 2, and 3 were thawed and stained with mesenchymal stem cell markers CD44-APC, CD90-PE, and CD105-Alexa405 along with a FITC cocktail containing lineage markers CD45, CD31, and SLA-DR Class II. Cells were pregated to exclude debris and doublets before gating on total live cells. a Frequency of CD90, CD44, and CD105 positive cells within QE1 cells of each donor Pig. b Frequency of CD90, CD44, and CD105 positive cells within QE2 cells for Pigs 1 and 3 and manually expanded Passage 3 cells of Pig 2 grown in tissue culture flasks. c Quantitative assessment of CD90, CD44, and CD105 positive cells within QE2 cells for Pigs 1 and 3 and manually expanded passage 3 cells of Pig 2 grown in tissue culture flasks