Fig. 3
Donor and passage dependent variability in metabolic activity of MSC. Reduction of Alamar blue dye was assessed and compared between groups to determine metabolic activity of cells over a period of 1 week. a At PreQE-P1, group comparisons show differences between Pig 1 and 3 over the course of the week (indicated as overall significance on right side of the line graph). Differences on individual days as shown on graph. b At passage 2, group comparisons show differences between Pig 2 and Pigs 1 and 3 over the course of the week (indicated as overall significance on right side of the line graph). Differences on individual days as shown on graph. c At passage 3, group comparisons shows differences between Pigs 1, 2 and 3 over the course of the week (indicated as overall significance on right side of the line graph). Differences on individual days as shown on graph. Values are presented as mean ± SEM. These are calculated based on 4 wells/day/donor at each of the passages analyzed. #Represents significant difference between Pigs 1 and 2, @represents significant difference between Pigs 2 and 3, *represents significant difference between 1 and 3; *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001