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Table 3 Sequences of primers used for RT-PCR and qRT-PCR

From: Clusterin silencing restores myoblasts viability and down modulates the inflammatory process in osteoporotic disease

Gene Primer sequences T annealing (°C)
CLU (RT-PCR) Sense: 5′-GTGCAATGAGACCATGATGG-3′ Antisense: 5′-CAGGTAGTGGTAGGTATCCT-3′ 55
sCLU (qRT-PCR) Sense: 5′-ATTCTCATCGCTTTGGAAGG-3′ Antisense: 5′-AGACATCAGGGGAGACTTTA-3′ 58
TGM2 (RT-PCR) Sense: 5′-GAGGAGCTGGTCTTAGAGAGG-3 ́ Antisense: 5′-CGGTCACGACACTGAAGGTG-3 ́ 62
β2-microglobulin (RT-PCR) Sense: 5′-CTGGAACGGTGAAGGTGACA-3′ Antisense: 5′-AAGGGACTTCCTGTAACAATGCA-3′ 60
GAPDH (qRT-PCR) Sense: 5′-ACGGATTTGGTCGTATTGG-3′ Antisense: 5′-GATTTTGGAGGGATCTCGC-3′ 60