Table 3 Sequences of primers used for RT-PCR and qRT-PCR
Gene | Primer sequences | T annealing (°C) |
|---|---|---|
CLU (RT-PCR) | Sense: 5′-GTGCAATGAGACCATGATGG-3′ Antisense: 5′-CAGGTAGTGGTAGGTATCCT-3′ | 55 |
sCLU (qRT-PCR) | Sense: 5′-ATTCTCATCGCTTTGGAAGG-3′ Antisense: 5′-AGACATCAGGGGAGACTTTA-3′ | 58 |
TGM2 (RT-PCR) | Sense: 5′-GAGGAGCTGGTCTTAGAGAGG-3 ́ Antisense: 5′-CGGTCACGACACTGAAGGTG-3 ́ | 62 |
β2-microglobulin (RT-PCR) | Sense: 5′-CTGGAACGGTGAAGGTGACA-3′ Antisense: 5′-AAGGGACTTCCTGTAACAATGCA-3′ | 60 |
GAPDH (qRT-PCR) | Sense: 5′-ACGGATTTGGTCGTATTGG-3′ Antisense: 5′-GATTTTGGAGGGATCTCGC-3′ | 60 |