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Table 3 Sequences of primers used for RT-PCR and qRT-PCR

From: Clusterin silencing restores myoblasts viability and down modulates the inflammatory process in osteoporotic disease

Gene Primer sequences T annealing (°C)
CLU
(RT-PCR)
Sense: 5′-GTGCAATGAGACCATGATGG-3′
Antisense: 5′-CAGGTAGTGGTAGGTATCCT-3′
55
sCLU
(qRT-PCR)
Sense: 5′-ATTCTCATCGCTTTGGAAGG-3′
Antisense: 5′-AGACATCAGGGGAGACTTTA-3′
58
TGM2
(RT-PCR)
Sense: 5′-GAGGAGCTGGTCTTAGAGAGG-3 ́
Antisense: 5′-CGGTCACGACACTGAAGGTG-3 ́
62
β2-microglobulin
(RT-PCR)
Sense: 5′-CTGGAACGGTGAAGGTGACA-3′
Antisense: 5′-AAGGGACTTCCTGTAACAATGCA-3′
60
GAPDH
(qRT-PCR)
Sense: 5′-ACGGATTTGGTCGTATTGG-3′
Antisense: 5′-GATTTTGGAGGGATCTCGC-3′
60