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Fig. 6 | Journal of Translational Medicine

Fig. 6

From: Clusterin silencing restores myoblasts viability and down modulates the inflammatory process in osteoporotic disease

Fig. 6

RT-PCR analysis of TGM2 expression and ICC analysis of acetyl histone H4 and CX3CR1 in OP and OA myoblasts transfected with CLU siRNA: a RT-PCR analysis of TGM2 expression. CLU silencing determined an increase of TGM2 expression only in OP myoblasts (siRNA vs scramble: p < 0.05); no modulation occurred in OA myoblasts. b ICC analysis of acetyl histone H4 in OP and OA myoblasts transfected with scramble and CLU siRNA. CLU silencing determined a strong down-regulation of positive nuclei for acetyl histone H4 expression only in OP myoblasts (siRNA vs scramble: p < 0.01); no significant modulation occurred in OA myoblasts. Nuclei are indicated by arrows; respectively, positive nuclei for staining in Scramble cells and negative nuclei in CLU siRNA treated cells. c ICC analysis of CX3CR1 in OP and OA myoblasts transfected with scramble and CLU siRNA. In this case, CLU silencing determined CX3CR1 cytoplasmic expression down-regulation in OP myoblasts (siRNA vs scramble: p < 0.05) and an increase of CX3CR1 cytoplasmic expression in OA myoblasts (siRNA vs scramble: p < 0.01). (*p < 0.05; **p < 0.01)

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