Fig. 3
Effect of recombinant CLU on OP and OA myoblasts: a effect of CLU conditioning on OP and OA myoblasts proliferation. Cells were seeded and 24 h after seeding recombinant CLU was added. Only OP myoblasts showed a decrease of proliferation after 6 days of treatment (CLU vs NT: p < 0.001) (NT: untreated; CLU: treated with recombinant CLU). b ICC analysis of NFKB expression in untreated and CLU treated OP and OA myoblasts. CLU conditioning determined a decrease of positive nuclei for NFKB expression, only in OP myoblasts (CLU vs NT: p < 0.001). Nuclei are indicated by arrows; respectively, positive nuclei for staining in NT cells and negative nuclei in CLU treated cells. c ICC analysis of acetyl histone H4 expression in untreated and CLU treated OP and OA myoblasts. In this case, CLU conditioning determined a significant increase of histone H4 acetylation levels in OP treated myoblasts (CLU vs NT: p < 0.05). No significant modulation occurred in OA cells. Nuclei are indicated by arrows; respectively, weakly positive nuclei for staining in NT cells and highly positive nuclei in CLU treated cells. (***p < 0.001; *p < 0.05; **p < 0.01)