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Table 1 Antibodies used for flow cytometry and rationale for marker selection

From: Quantitation of progenitor cell populations and growth factors after bone marrow aspirate concentration

 

Rationale for analysis

Specific antibody

Fluoro-chrome

Clone

Manufacturer

Mandatory

Marker presence/absence identify MSCs (include “ISCT markers”)

Optional

Marker presence refine MSC subpopulations phenotypes

Anti-human CD29

PE

MAR4

BD Biosciences

Present in vitro (ISCT consensus) [9]

 

Anti-human CD44

APC

DB105

Miltenyi Biotec

(Bergisch-Gladbach, Germany)

Present in vitro [5]

 

Anti-human CD73

APC

AD2

Biolegend

(San Diego, CA, USA)

Present in vitro (ISCT consensus) and in vivo [4, 5, 28]

 

Anti-human CD90

APC

5E10

BD Biosciences

Present in vitro (ISCT consensus) and in vivo [5, 6, 28]

 

Anti-human CD90

PE

5E10

BD Biosciences

 

Anti-human CD105

purified

266

BD Bioscience

Present in vitro (ISCT consensus) [28]

 

Anti-human CD34

FITC

581

Molecular Probes Life Technologies

(Carlsbad, CA, USA)

Absent in vitro (ISCT consensus) [28]; identifies HSC

 

Anti-human CD45

FITC

HI30

BD Biosciences

Absent in vitro (ISCT consensus) [28]; identifies WBC and HSC

 

Anti-human CD10

PE

HI10A

BD Biosciences

 

In vitro and in vivo [4, 5]

Anti-human CD119

PE

GIR-208

BD Biosciences

 

In vitro [5]

Anti-human CD271

PE

ME20.4-1.H4

Miltenyi Biotec

 

In vitro and in vivo [4,5,6]

Anti-human GD2

purified

14.G2A

BD Bioscience

 

In vitro and in vivo [4, 5]

Goat anti-mouse

PE

Polyclonal

BD Bioscience

  

IgG1

FITC

MOPC-21

BD Biosciences

  

IgG1

PE

MOPC-21

BD Biosciences

  

IgG1

APC

MOPC-21

BD Biosciences