Rationale for analysis | |||||
---|---|---|---|---|---|
Specific antibody | Fluoro-chrome | Clone | Manufacturer | Mandatory Marker presence/absence identify MSCs (include “ISCT markers”) | Optional Marker presence refine MSC subpopulations phenotypes |
Anti-human CD29 | PE | MAR4 | BD Biosciences | Present in vitro (ISCT consensus) [9] | |
Anti-human CD44 | APC | DB105 | Miltenyi Biotec (Bergisch-Gladbach, Germany) | Present in vitro [5] | |
Anti-human CD73 | APC | AD2 | Biolegend (San Diego, CA, USA) | ||
Anti-human CD90 | APC | 5E10 | BD Biosciences | ||
Anti-human CD90 | PE | 5E10 | BD Biosciences | ||
Anti-human CD105 | purified | 266 | BD Bioscience | Present in vitro (ISCT consensus) [28] | |
Anti-human CD34 | FITC | 581 | Molecular Probes Life Technologies (Carlsbad, CA, USA) | Absent in vitro (ISCT consensus) [28]; identifies HSC | |
Anti-human CD45 | FITC | HI30 | BD Biosciences | Absent in vitro (ISCT consensus) [28]; identifies WBC and HSC | |
Anti-human CD10 | PE | HI10A | BD Biosciences | ||
Anti-human CD119 | PE | GIR-208 | BD Biosciences | In vitro [5] | |
Anti-human CD271 | PE | ME20.4-1.H4 | Miltenyi Biotec | ||
Anti-human GD2 | purified | 14.G2A | BD Bioscience | ||
Goat anti-mouse | PE | Polyclonal | BD Bioscience | ||
IgG1 | FITC | MOPC-21 | BD Biosciences | ||
IgG1 | PE | MOPC-21 | BD Biosciences | ||
IgG1 | APC | MOPC-21 | BD Biosciences |