Fig. 4

FNDC5 deficiency enhanced palmitate-induced inflammation and NOX4 expression in primary cardiomyocytes (CMs). a Effects of palmitate (PA, 400 μM) treatment on Tnf-α, Il1b and Il6 mRNA levels in CMs of WT and FNDC5^−/− mice. b Effects of PA treatment on NOX2 and NOX4 protein level in CMs of WT and FNDC5^−/− mice. The measurement was 24 h after PA treatment for measuring the mRNA levels or protein levels. PA: Palmitate; Veh: Vehicle; F^−/−: FNDC5^−/−. Values are mean ± SEM. *P < 0.05 vs. Veh. ^†P < 0.05 vs. CMs-WT. n = 3