Fig. 2

FNDC5 deficiency aggravated HFD-induced inflammation in heart. a Cardiac Tnf-α, Il1b and Il6 mRNA levels determined with qPCR. b Phosphorylation levels of p38 and ERK in heart determined with Western blot. c NFκB activation in heart. N-p65: p65 in nucleus; C-p65: p65 in cytoplasm. Values are mean ± SEM. *P < 0.05 vs. WT. †P < 0.05 vs. Ctrl. n = 3