Fig. 1

FNDC5 deficiency worsened HFD-induced cardiac hypertrophy in mice. WT and FNDC5^−/− mice were fed with mouse chow diet (Ctrl) and high fat diet (HFD) for 20 weeks. The measurements were carried out at the end of 20 weeks after Ctrl or HFD feeding. a Expression of FNDC5 protein in heart determined with Western blot. b, c Body weight (BW), left ventricular (LV) mass and left ventricular weight/body weight (LVW/BW). d The mRNA expression of cardiac hypertrophy markers in heart. e Representative sections of H&E staining show the cardiomyocyte area in heart. Values are mean ± SEM. *P < 0.05 vs. WT. ^†P < 0.05 vs. Ctrl. n = 6