Fig. 1

KA supplement protected mice from IR induced renal injury and metabolic disorders. a The experiment scheme in vivo. Mice were divided into 3 groups: Sham, IR + Nacl (IR surgery + 0.9% Nacl solvent), IR + KA (IR + KA dissolved in 0.9% Nacl: 1000 mg/kg/d). LPD was administrated after IR surgery, at the same time with KA for 28 days. b, c PAS staining showed a mass of tubular expansions, casts and loss of tubular brush border in IR + Nacl group. With the treatment of KA, tubular expansions were ameliorated and casts were reduced. d, e LTL staining showed IR caused more than 2.0-folds decrease of healthy tubules, KA preserved nearly 1.8 folds increased remnant healthy tubules compared to IR + Nacl group. f, g The detection of Kim-1 showed a same phenomenon as LTL. An extensive injure renal tubules were founded in IR + Nacl group. In IR + KA group, the number of kim-1 positive tubules decreased. h Serum BUN and plasma TC and TG were detected by using kits. KA reduced IR induced enhancement of BUN and TG. Otherwise, KA showed a slight decrease in IR induced TC disorder, the concentration of TC in IR + Nacl was 4.74 mmol/L while in IR + KA was 4.19 mmol/L. Scale bars: 50 μm. Data were presented as mean ± SD, *p < 0.05, **p < 0.01 (unpaired nonparametric t-test); n = 4–6/group