Table 1 Primer sequences for PCR
From: Cartilage progenitor cells combined with PHBV in cartilage tissue engineering
Gene | Accession numbers | Primer | Product (bp) |
|---|---|---|---|
Aggrecan | NM_001135 | Sense 5′-GGGGAATCTTCTGGCATTAA-3′ | 381 |
Antisense 5′-CGTTGGAGCCTGGGTT-3′ | |||
SOX-9 | NM_000346.4 | Sense 5′-GGCTCGGACACAGAGAACAC-3′ | 195 |
Antisense 5′-GTGCGGCTTATTCTTGCTCG-3′ | |||
COL II a1 | NM_001844.5 | Sense 5′-TGCTGCTGACGCTGCTC-3′ | 294 |
Antisense 5′-GTTCTCCTTTCCTGTCCCTTTG-3′ | |||
VEGF | NM_001025366.2 | Sense 5′-CATCTTCAAGCCGTCCTGTGT-3′ | 142 |
Antisense 5′-TCCTATGTGCTGGCCTTGGT-3′ | |||
β-Actin | NM_001101.5 | Sense 5′-ACATCAAGGAGAAGCTCTGCTACG-3′ | 366 |
Antisense 5′-GAGGGGCGATGATCTTGATCTTCA-3′ |