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Table 4 Associations between the circulating biomarkers and the histopathological findings

From: Experimental non-alcoholic steatohepatitis in Göttingen Minipigs: consequences of high fat-fructose-cholesterol diet and diabetes

Elements

ORO-VIS

PSR-VIS

Iba1-VIS

Steatosis score

Fibrosis score

Inflammation score

CA score

PAS score

Biomarkers

 TC

< 0.0001 (0.41)a

0.01 (0.17)a

< 0.0001 (0.45)a

0.05 (0.16)a

0.2 (0.14)

0.02 (0.26)a

< 0.0001 (0.56)a

0.3 (0.10)

 TG

0.001 (0.28)a

0.01 (0.17)a

0.0007 (0.28)a

0.2 (0.10)

0.04 (0.22)a

0.009 (0.29)a

< 0.0001 (0.49)a

0.4 (0.09)

 ALP

0.003 (0.23)a

0.002 (0.25)a

0.0006 (0.29)a

0.11 (0.12)

0.04 (0.22)a

0.008 (0.30)a

< 0.0001 (0.55)a

0.03 (0.24)a

 ALT

0.2 (0.06)

0.4 (0.02)

0.005 (0.20)a

0.02 (0.21)a

0.6 (0.05)

0.5 (0.07)

0.1 (0.15)

0.1 (0.15)

 AST

0.02 (0.16)a

0.01 (0.17)a

0.2 (0.05)

0.1 (0.13)

0.01 (0.27)a

0.2 (0.13)

0.03 (0.23)a

0.9 (0.02)

 AST:ALT

0.0005 (0.31)a

0.0001 (0.35)a

0.006 (0.20)a

0.02 (0.20)a

< 0.0001 (0.47)a

0.008 (0.30)a

0.0004 (0.42)a

0.5 (0.07)

 GLDH

0.001 (0.27)a

0.007 (0.19)a

0.003 (0.22)a

0.2 (0.10)

0.002 (0.35)a

0.03 (0.23)a

0.0002 (0.44)a

0.7 (0.05)

 CRP

0.7 (0.01)

0.6 (0.01)

0.2 (0.04)

0.5 (0.03)

0.8 (0.03)

0.9 (0.02)

0.7 (0.04)

1 (0.00)

  1. All response variables were transformed for normal distribution of residuals
  2. ALP alkaline phosphatase, ALT alanine transaminase, AST aspartate transaminase, CRP C-reactive protein, CA cytoplasmic alterations, GLDH glutamate dehydrogenase, Iba1-VIS quantification of macrophages on Iba1 immunohistochemical stain, ORO-VIS quantification of lipid on oil red O stain, PAS positive periodic acid Schiff stain for glycogen, PSR-VIS quantification of collagen on picro-sirius red stain, TC total cholesterol, TG triglycerides
  3. Data presented as p-value (R2)
  4. aSignificant result