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Table 2 Overview of elements in the qualitative histopathological assessment

From: Experimental non-alcoholic steatohepatitis in Göttingen Minipigs: consequences of high fat-fructose-cholesterol diet and diabetes

Elements from human diagnostics

Score

Type/localization

Steatosis (NAS)

0: < 5%

1: 5–33%

2: > 33–66%

3: > 66%

Macrovesicular, microvesicular or mixed

Zone 1, 2, 3

Fibrosis (NAS)

0: None

1: Perisinusoidal or periportal

2: Perisinusoidal and portal/periportal

3: Bridging

4: Cirrhosis

Subgroups of score 1:

1A: Mild, zone 3, perisinusoidal

1B: Moderate, zone 3, perisinusoidal

1C: Portal/periportal

Inflammatory foci per 200× field, mean of 3 fields (NAS)

0: 0

1: < 2

2: 2–4

3: > 4

Lobular or portal

Hepatocellular ballooning

0: None

1: Few balloon cells

2: Many cells/prominent ballooning

Hydropic degeneration

Mallory–Denk bodies

0: None to rare

1: Many

 

Other elements

Score

Localization

Cytoplasmic alterations

0: −

1: +

2: ++

3: +++

Zone 1, 2, 3

PAS positive staining

0: −

1: +

2: ++

3: +++

Zone 1, 2, 3

  1. For each element scoring, localization and/or type were recorded
  2. NAS Modified from the NAFLD activity score (NAS) [22], PAS Periodic acid Schiff (glycogen staining)