Fig. 2

T-lymphocytes modulation by heparan sulfate (HS) and syndecan-1 (syn-1). Profile of sulfated glycosaminoglycans (GAG) synthesized by circulating lymphocytes. Circulating lymphocytes were maintained in culture for 4 h at 37 °C and GAG were identified and quantified by agarose gel electrophoresis after [³⁵S]-sulfate labeling. a T-lymphocytes from a breast cancer patient; b T-lymphocytes of a woman not affected by cancer (healthy woman); c co-culture of T-lymphocytes from a healthy woman and MCF-7 cells; 1, cellular fraction; 2, medium; 3, digestion using chondroitinase AC; 4, digestion with heparitinase II. d The values represent digital quantification of immunocytochemistry assays performed in triplicates using lymphocytes obtained from three different healthy donors. T-Lymphocytes, circulating lymphocytes collected from a woman not affected by neoplasia; Lymphocytes were previously incubated for 4 h at 37 °C as indicated; + co-culture medium, in the presence of co-culture medium; + co-culture medium with anti-syn1, with co-culture medium in the presence of anti-syndecan-1 antibody (MCA681-CD138); + co-culture medium with HTase II, with co-culture medium in the presence of the enzyme heparitinase II. *p < 0.0001 and **p < 0.05 Kruskal–Wallis test with Dunn auxiliary test