Fig. 5

5-Aza-induced demethylation increases ZCCHC13 expression in human liver cancer cells. a Results of MSP, b RT-qPCR, c RT-PCR and d Western blot analysis showing that 5-Aza attenuated the methylation of the ZCCHC13 promoter in HepG2 cells. U: Unmethylated MSP amplicons; M: methylated MSP amplicons. GAPDH was used as an internal control. The results are presented as the mean ± SD of three independent experiments (*p < 0.05 and **p < 0.01)