Fig. 4

DNA methylation status of ZCCHC13 in human liver cancer cell lines and specimens. a Methylation status of 8 CpG dinucleotides in the ZCCHC13 promoter in LO2, HepG2, and Huh7 cells. Columns indicate alterations in the methylation of CpG sites contained in the BSP amplicon based on 10 cloned sequences for each cell line. b ZCCHC13 methylation status in 10 pairs of tumor and normal samples. Black dot, methylated CG; white dot, unmethylated CG. c Methylation data were obtained from TCGA through the MethHC database for pairs of tumor (n = 204) and (n = 65) normal tissues. Beta values range from 0 to 1, with 0 representing the unmethylated DNA and 1 representing the completely methylated DNA