Fig. 2
ZCCHC13 expression pattern in HCC specimens. a ZCCHC13 immunoreactivity was detected using immunochemistry at a high magnification of ×400. Scale bar, 20 μm. b Western blot showing the levels of the ZCCHC13 protein in 4 independent samples; T, tumor tissue; N, normal tissue. c Statistical analysis of the mean IOD for ZCCHC13-positive staining in tissue sections according to the following formula: mean IOD = IOD/area of the tumor section. Three images from each slide were assessed. The results are presented as the mean ± SD of three images from each slide. d Quantification of Western blot results for the ZCCHC13 protein in HCC specimens (****p < 0.0001)