Fig. 1
From: Longitudinal heterogeneity in glioblastoma: moving targets in recurrent versus primary tumors
Target investigation by immunohistochemistry. a Typical examples of strongly immunoreactive targets (scale bar 50 µm). b Shifting target expression as revealed by quantitative scoring (left panel), illustrated by changes per target (right panel). The thickness of lines indicates the number of patients. If the status changed between primary and recurrent tumor tissue, the percentage of affected patients was indicated in circles in the graph (numbers rounded). For PDGFR-β, FGFR-2, and FGFR-3 the numbered boxes represent the portion of positively labeled tumor cells: box 0 = negative; box 1 ≤ 10%; box 2 = 10–90%; box 3 ≥ 90%. Due to spatial/intratumoral inhomogeneous staining results of phosphor-mTORSer2448: box 0 = negative; box 1 = smaller groups, but < 50% of tumor cells; box 2 = major groups, > 50% of tumor cells; box 3 = nearly all tumor cells positive