Fig. 5

TSP4-BMSCs enhanced angiogenesis by promoting MMP2 and MMP9 expression in the ischemic brain penumbra of rats. a, b Immunofluorescence staining of MMP2 (green) (scale bar 100 μm) is shown, and the relative expression of VEGF was quantified. Data are expressed as the mean ± SD. **p < 0.01. c, d Immunofluorescence staining of MMP9 (green) (scale bar, 100 μm) is shown, and relative expression of MMP9 was quantified. Data are expressed as the mean ± SD. **p < 0.01. The experiment was repeated 3 times, and representative pictures are shown. Phenotype identification of BMSCs and TSP4-BMSCs. a BMSCs from passage 3 were incubated with fluorescence-conjugated antibodies, including CD90-PerCP, CD45-Alexa, CD34-PE and PBS. The positive expression of CD90 was 99.76%, and the negative identification of CD45 and CD34 was 2.74 and 3.55%, respectively. b TSP4-BMSCs were incubated with fluorescence-conjugated antibodies, including CD90-PerCP, CD45-Alexa, CD34-PE and PBS. The positive expression of CD90 was 99.56%, and the negative identification of CD45 and CD34 was 4.97 and 5.97%, respectively