Fig. 1

Construction of the PLV-Easy-GFP-TSP4 plasmid and expression of the TSP4 target protein. a Schematic illustration of the structure of PLV-Easy-GFP-TSP4. b SalI and HindIII double digestion of two recombinant plasmids into 7256 and 1720 bp fragments. The positions of the 7000 and 2000 bp size markers are indicated to the left of the panel. c The plasmids psPAX2, pMD.2G and PLV-Easy-GFP-TSP4 were transfected into 293T cells (green) in the appropriate proportions. The positive rate of transfection was 94.73% ± 0.0126. d The PLV-Easy-GFP-TSP4 lentivirus infected BMSCs (green). The positive rate of infection was 76.95% ± 0.0278. e Fluorescent staining of PLV-Easy-GFP-TSP4-BMSCs and BMSCs (nucleus in blue, cytoskeleton in red, and TSP4 protein in green). f The TSP4 content in the supernatant of BMSCs was detected by ELISA. Data are expressed as the mean ± SD. **p < 0.01. g Western blot to determine the expression of TSP4 secreted by BMSCs in the two groups (BMSCs and TSP4-BMSCs). h Western blot results of TSP4 and quantitative analysis. Data are expressed as the mean ± SD. **p < 0.01