Fig. 6

Blockade of EP2 enhances the immune response in ACLF. Isolated PBMCs from at least 9 subjects per group were stimulated with LPS in the presence of AH6809 (150 μM) or DMSO for 72 h in vitro. The impact of EP2 blockade on cytokine production was determined by flow cytometry (intracellular staining) and multi-plex human cytokine assay (supernatants). a Percentage of cytokines producing PBMCs. b Concentration of cytokines in the supernatants. c One hundred microliters of peripheral blood from 8 subjects per group was stimulated with E. coli in the presence of AH6809 (150 μM) or DMSO for 30 min in vitro. The effect of EP2 blockade on ROS production of monocytes and neutrophils was determined by flow cytometry. d One hundred microliters of peripheral blood from HC (n = 10), CHB (n = 12) and ACLF (n = 10) was incubated with AF488-E. coli in the presence of AH6809 (150 μM) or DMSO for 30 min in vitro. The effect of EP2 blockade on the phagocytosis of monocytes and neutrophils was determined by flow cytometry. Statistical analyses were performed using the Wilcoxon signed-rank test. *p < 0.05, **p < 0.01, ***p < 0.001. ns, not statistically significant