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Fig. 4 | Journal of Translational Medicine

Fig. 4

From: Circular RNA hsa_circ_0055538 regulates the malignant biological behavior of oral squamous cell carcinoma through the p53/Bcl-2/caspase signaling pathway

Fig. 4

High expression of hsa_circ_0055538 inhibits OSCC cell migration and invasion. a, b Wound healing assays were performed using a SCC9 and b CAL27 cells infected with the empty vector control or lentivirus harboring hsa_circ_0055538. The scratch area was measured at 0 and 24 h, and the percentage of closure at 24 h was calculated. c, d Transwell assays were performed to quantify the migration and invasion ability of SCC9 and CAL27 cells infected with the empty vector control or lentivirus harboring hsa_circ_0055538. c Cells were seeded into the upper chamber (uncoated); after 24 h, those that crossed to the lower chamber were used for imaging and quantification: SCC9 (top), CAL27 (bottom). The proportion of migrating cells was quantified using SCC9 (left) and CAL27 (right) cells. d Cells were seeded into the Matrigel-coated upper chamber; after 48 h, those that passed across the coated chamber were used for imaging and quantification: SCC9 (top), CAL27 (bottom). The proportion of invading cells was quantified in SCC9 (left) and CAL27 (right) cells. Data are presented as mean ± SEM of three independent experiments. Student’s t-test, ***P < 0.001, **P < 0.01

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