Fig. 5

Cytotoxicity and IFN stability. Apheresis and counter-flow elutriation was performed on four donors. A final product was generated combining the monocytes with IFNs at the second dose level (a, b) and the fourth dose level (c, d). The products were assayed for cytotoxicity to OVCAR3 ovarian cancer cells. Time 0 h product (black bars), product stored 4 h at room temperature (light grey), IFNs alone (dark grey), and cell medium supernatant without IFNs (white bar) were measured. e IFN stability was measured after incubation at room temperature for 0, 2, 4, and 5 h; initial (D1) and overnight (4c) monocyte supernatants are used as controls, for cytotoxicity with OVCAR3 cells