Fig. 3

Flow cytometric analysis and quantification of immune subtypes in the four fractions of counter-flow elutriation. a A condensed work flow of flow cytometry of of cells from counter flow elutriation, with a focus on the monocyte population (red box). b Percentages of immune subtypes in all fractions from healthy donor 1. c Percentages of immune subtypes in all fractions from healthy donor 2. d Proportion of each immune subtype by fraction of elutriation for healthy donor 1. e Proportion of immune subtypes by fraction of elutriation for healthy donor 2 (complete work-flow analysis in Additional file 1: Table S3, Additional file 2: Figure S1, Additional file 3: Figure S2, Additional file 4: Figure S3 and Additional file 5: Figure S4)