Fig. 2

Quantification of cell numbers and viability. Apheresis followed by counter-flow elutriation was performed on 4 healthy donors. a Total nucleated cells and total monocytes from the bullk apheresis shown for all donors as total cell number. b Total monocytes from the bulk apheresis. c Total Nucleated Cells in the RO fraction. d Total monocytes in the RO fraction. e Recovery of total nucleated cells from overnight storage was measured. f Viability of the final product monocytes at 0 h (black bars) and 4 h (grey bars) was measured. g Kinetics monocyte viability the on day 1 (black bars), day 2 (grey bars) and the final product were measured. h Total nucleated cell count was performed on the final product at 0 h (black bars), 2 h (grey bars), and 4 h (white bars)