Fig. 6
From: Testosterone induces renal tubular epithelial cell death through the HIF-1α/BNIP3 pathway
HIF-1α regulates BNIP3 expression and cell death. a The levels of HIF-1α, HIF-2α and BNIP3 in cells treated with the HIF-1α inhibitor 40083 and/or HIF-2α inhibitor YC-1 and subsequently stimulated with testosterone (20 nM) were determined using western blotting. Cells were pretreated with 40083 (50 μM) and YC-1 (100 μM) for 30 min. b The apoptosis and necrosis of these cells were also assessed using flow cytometry. Data are presented as the mean values each group (mean ± SD)