Fig. 5
From: Testosterone induces renal tubular epithelial cell death through the HIF-1α/BNIP3 pathway
Testosterone induces renal TECs death through the caspase-independent pathway. a Levels of the BNIP3, Bcl-2, Bax pro-caspase-9, caspase-9, pro-caspase-8, cleaved-caspase-8, cleaved-caspase-3 and cytochrome C proteins in HK-2 and TCMK-1 cells transfected with or without the BNIP3 siRNA and stimulated with testosterone (20 nM) were examined by western blotting. b The ΔΨm was detected by determining the ratio of green/red fluorescence of JC-1. Data are presented as values for each group (mean ± SD)