Fig. 4
From: Testosterone induces renal tubular epithelial cell death through the HIF-1α/BNIP3 pathway
BNIP3 knockdown reverses testosterone-induced apoptosis and necrosis. a Forty-eight hours after transfection with siRNAs, HK-2 and TCMK-1 cells were further exposed to 20 nM testosterone for 24 h. Apoptosis and necrosis were detected by flow cytometry using an Annexin V-PI assay. b The TUNEL assay was performed to detect apoptotic cells. Data are presented as the mean values for each group (mean ± SD)