Fig. 2From: TZAP plays an inhibitory role in the self-renewal of porcine mesenchymal stromal cells and is implicated the regulation of premature senescence via the p53 pathwayTZAP expression increased with pMSC aging (P2, P6 and P10). The rates of cell growth and EdU incorporation were assayed by a CCK8 (a) assay and an EdU assay (b), respectively. Bar = 100 μm. c Bar = 100 μm. The osteogenic and adipogenic potential of pMSCs at different passages was assessed by alizarin red S staining and oil red O staining, respectively, after induction of differentiation. d The expression of bone markers (Alp, Runx2, and Col1a1) and adipogenic markers (Cebpa) was assessed by qRT-PCR after adipogenic and osteogenic induction, respectively, in pMSCs at different passages. e Bar = 100 μm. Comparison of the SA-β-gal staining profiles of pMSCs at different passages. f Protein levels of TZAP, P16INK14 (P16) and P21 in pMSCs at different passages were compared through western blot analysis. g The mRNA levels of TZAP, p16INK14 (p16) and p21 in pMSCs at different passages were detected by qRT-PCR. All data are represented as the mean ± SEM. n = 3. *p < 0.05; **p < 0.01; #p < 0.001, tested by one-way ANOVABack to article page