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Fig. 2 | Journal of Translational Medicine

Fig. 2

From: TZAP plays an inhibitory role in the self-renewal of porcine mesenchymal stromal cells and is implicated the regulation of premature senescence via the p53 pathway

Fig. 2

TZAP expression increased with pMSC aging (P2, P6 and P10). The rates of cell growth and EdU incorporation were assayed by a CCK8 (a) assay and an EdU assay (b), respectively. Bar = 100 μm. c Bar = 100 μm. The osteogenic and adipogenic potential of pMSCs at different passages was assessed by alizarin red S staining and oil red O staining, respectively, after induction of differentiation. d The expression of bone markers (Alp, Runx2, and Col1a1) and adipogenic markers (Cebpa) was assessed by qRT-PCR after adipogenic and osteogenic induction, respectively, in pMSCs at different passages. e Bar = 100 μm. Comparison of the SA-β-gal staining profiles of pMSCs at different passages. f Protein levels of TZAP, P16INK14 (P16) and P21 in pMSCs at different passages were compared through western blot analysis. g The mRNA levels of TZAP, p16INK14 (p16) and p21 in pMSCs at different passages were detected by qRT-PCR. All data are represented as the mean ± SEM. n = 3. *p < 0.05; **p < 0.01; #p < 0.001, tested by one-way ANOVA

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