Fig. 1

Identification of pMSCs isolated from the bone marrow of Tibet minipigs. a Bar = 100 μm. Fibroblast-like morphology of pMSCs isolated from the bone marrow of Tibet minipigs. b Bar = 100 μm. Trilineage differentiation potential of pMSCs demonstrated at P3. Oil red O, alizarin red S, and Alcian blue staining were used to determine the adipogenic, osteogenic, and chondrogenic differentiation of pMSCs, respectively. c FACS analysis of pMSC-specific markers on the cell surface at P3, and pMSC identity was confirmed by the detection of positive results for CD105, CD90, and CD73 and negative results for CD45 and CD34. The control cells were stained with a nonimmunoreactive isotype control antibody. Top row, images of the isotype controls. Bottom row, images of the experimental flow cytometric analyses