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Table 5 Reproducibility and sensitivity of the one-step Taqman PCR assay

From: Bioinformatics-based discovery of the urinary BBOX1 mRNA as a potential biomarker of diabetic kidney disease

 

CT1

CT2

CT3

CV (%)

500 ng total RNA/10 µl

 BBOX1

25.29

25.54

25.38

0.49

 B2M

15.51

15.58

15.52

0.24

50 ng total RNA/10 µl

 BBOX1

28.19

28.26

28.24

0.10

 B2M

18.84

18.81

18.85

0.09

5 ng total RNA/10 µl

 BBOX1

31.55

31.43

31.42

0.19

 B2M

22.25

22.29

22.2

0.17

0.5 ng total RNA/10 µl

 BBOX1

33.87

33.86

34.06

0.27

 B2M

25.74

25.73

25.71

0.05

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Journal of Translational Medicine

ISSN: 1479-5876

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