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Table 5 Reproducibility and sensitivity of the one-step Taqman PCR assay

From: Bioinformatics-based discovery of the urinary BBOX1 mRNA as a potential biomarker of diabetic kidney disease

  CT1 CT2 CT3 CV (%)
500 ng total RNA/10 µl
 BBOX1 25.29 25.54 25.38 0.49
 B2M 15.51 15.58 15.52 0.24
50 ng total RNA/10 µl
 BBOX1 28.19 28.26 28.24 0.10
 B2M 18.84 18.81 18.85 0.09
5 ng total RNA/10 µl
 BBOX1 31.55 31.43 31.42 0.19
 B2M 22.25 22.29 22.2 0.17
0.5 ng total RNA/10 µl
 BBOX1 33.87 33.86 34.06 0.27
 B2M 25.74 25.73 25.71 0.05