Fig. 2

Unbalance between osteoclast and osteoblast and high adipoR1 expression were observed in AIS cancellous bone. a Total RNA were extracted from two group. The relative expression level of RANKL, OPG, RANK, adiponectin, adipoR1, adipoR2, ALP, osterix, runx2 were detected by quantitative real-time PCR. The Y axis represents the fold change in transcript levels in three group. The control group was set to 1.0. b, c Protein level of RANKL, OPG, RANK, adipoR1 were measured by western blotting. The Y axis represents the fold change and control were set to 1.0. d TRAP staining of facet joint in AIS and control group. Scale bar: 50 μm. e Quantitative analysis of the number of TRAP⁺ cells. BS bone surface, OCs osteoclast. f, h Representative images of immunohistochemical staining for OCN and adipoR1. g, i Quantitative analysis of the mean intensity for positively stained areas. Data are shown as the mean ± SD. One way ANOVA was use to tested, when p value > 0.05, there was no significant difference in all the groups; when p value < 0.05, then using bonferroni correction to test each two groups. *p < 0.0167 Bonferroni correction test between control and AIS concave. Bonferroni correction test between control and AIS convex. ^#p < 0.0167 Bonferroni correction test between AIS concave and AIS convex