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Fig. 5 | Journal of Translational Medicine

Fig. 5

From: The biological behavior optimization of human periodontal ligament stem cells via preconditioning by the combined application of fibroblast growth factor-2 and A83-01 in in vitro culture expansion

Fig. 5

Effect of different preconditioning strategies on stemness maintenance of PDLSCs. Fourth generation PDLSCs preconditioned by various treatments were cultured in the same culture medium for 3 and 7 day. Cells were collected for RT-PCR. The gene expression of c-Myc and Nanog was measured on Light Cycler Roche 480 II Real-Time PCR System. A Comparision of gene expression of c-Myc and Nanog among different groups after 3 day culture. B Comparision of gene expression of c-Myc and Nanog among different groups after 7 day culture. (No matching letters (e.g.: a, b, c, d) above any two column charts represents a significant difference between these two groups (p < 0.05), while the appearance of any matching letters (e.g.: a, a) means that there is no significant difference among groups (p > 0.05). Data are expressed as mean ± SD)

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