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Fig. 1 | Journal of Translational Medicine

Fig. 1

From: High throughput sequencing of T-cell receptor repertoire using dry blood spots

Fig. 1

The work flow of DBS based TCR repertoire discovery. Finger-prick blood is collected onto the filter card and extracted RNA from DBS is used as a template for amplification. The entire PCR amplification and library preparation are automated in a closed cassette. Post amplification, libraries are pooled and quantified then sequenced by NGS. Sequencing data is analyzed by iRweb’s bioinformatics platform

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