Fig. 3

Comparable B cell subsets and serum immunoglobulin levels between WT and Lrrk2^−/− Mice. a Splenocytes were collected from WT and Lrrk2^−/− mice. B cell subsets in the periphery were analyzed by flow cytometry, including total B cells (B220⁺ lived cells, upper), mature B cells (B220⁺CD93⁻, middle), transition (TS) B cells (B220⁺CD93⁺, middle), marginal zone (MZ) B cells (B220⁺CD93⁻CD23⁻IgM⁺, low), and follicular (FO) B cells (B220⁺CD93⁻CD23⁺ IgM^low/−, low). b Statistic percentages of total B cells, mature B cells and TS B cells in the spleens of WT and Lrrk2^−/− mice. c Statistic percentages of MZ and FO B cells in the spleens of WT and Lrrk2^−/− mice. d Sera from WT and Lrrk2^−/− mice were subjected to the determination of total IgG and IgM by ELISA as described in “Methods”. OD₄₅₀ value was detected to represent IgG (left) and IgM (right) levels in the serum of the mice. Each group had at least six mice for the analysis. ns not significant