Fig. 2

Macrophages depletion in the tissue expansion model following clodronate liposomes injection regimen. a Skins were injected with clodronate liposomes (Clo-lipo) or PBS liposomes control (PBS-lipo) at day 0 immediately after expander implantation, day 3 and day 10. Skin tissues were collected at day 7, day 14 and day 35. b Clo-lipo or PBS-lipo were subcutaneously injected on the right or left side of the tattooed expanded skin (1.5 cm × 1 cm) at indicated days. c Skin sections were stained with anti-CD68 (red) to visualize macrophages in Clo-lipo and PBS-lipo treated expanded skin at day 7, day 14 and day 35. d Morphometric quantification of macrophages in the expanded skin at indicated time points following expander implantation (at least 5 animals at each time point). *p ≤ 0.05, ****p ≤ 0.0001