Fig. 3

Comparison of NanoVelcro and CellSearch in CTC capture ability. a Immunofluorescence staining of CTC immobilized on NanoVelcro substrates. Typical micrographs of CTC and WBCs were immobilized on a NanoVelcro substrate. Scale: white bars correspond to 10 μm. b The enumerated CTC by NanoVelcro from 1.0 ml blood and by CellSearch from 7.5 ml in NSCLC adenocarcinoma patients at their first visit (Normalized to 1.0 ml scale for comparison). Red represents the enumerated CK+/CD45− CTC by NanoVelcro in patient blood; Green represents CellSearch counts CTC. c Linear regression plots were computed for CTC counts obtained by CellSearch and NanoVelcro (Normalized to 7.5 ml scale for comparison). d Correlation analysis between pTNM stage with NanoVelcro and CellSearch CTC counts respectively in all NSCLC adenocarcinoma patients (Normalized to 1.0 ml scale for comparison)