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Fig. 2 | Journal of Translational Medicine

Fig. 2

From: Three-dimensional nanostructured substrates enable dynamic detection of ALK-rearrangement in circulating tumor cells from treatment-naive patients with stage III/IV lung adenocarcinoma

Fig. 2

NanoVelcro device configuration and work mechanism. a NanoVelcro CTC Chip is composed of an overlaid PDMS-based chaotic mixer, a patterned silicon nanowire (SiNW) substrate, and a multilayer chip holder to assemble both functional components together. b Herringbone features introduce a helical flow in the microchannel facilitating the immunological recognition between CTC and anti-EpCAM coated SiNW substrate. c Schematic representation of an immunofluorescence protocol developed for identification of CTC (CK+/CD45−/DAPI+) from non-specifically captured WBCs (CK−/CD45+/DAPI+) and cell debris. d Silanation reaction were employed to covalently link streptavidin onto the SiNW substrate, allowing conjugation of biotinylated anti-EpCAM prior to CTC detection studies

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