Fig. 4

Western blotting analyses of transcription factor TFEB and βcatenin, performed on whole lysates from HBL (wtBRAF) and hmel1 and M3 (V600BRAF) metastatic melanoma cell lines. a Bar graph shows quantification of TFEB western blotting densitometric profiles normalized for GAPDH as loading control and HBL as control cell line. b Bar graph shows quantification of β-catenin by densitometric profiles normalized for β-actin used as loading control and HBL as control cell line. Values are mean ± SEM of three independent experiments. Significance was calculated with Student’s t test; *p < 0.05, **p < 0.005 vs HBL