Fig. 4

In vivo experimental design. Two in vivo experiments were performed. a In experiment 1 we used healthy C57BL/6. Mice were injected IV with either saline, MBs, hAd-GFP, MBs(hAd-GFP), or MBs(hAd-GFP) + US. Blood samples were collected 2 h post-injection to determine inflammatory cytokine response. After 4 weeks, mice were re-injected and 4 weeks later, mice were sacrificed and blood and organs were collected. b In experiment 2 we used C57BL/6 mice injected subcutaneously with 5 × 10⁶ TRAMP-C2 cells. When syngeneic tumor grafts were established (approximately 3 weeks post-injection of cancer cells, and with tumor volumes between 50 and 100 mm³) mice were injected IV with either saline, MBs, hAd-GFP, MBs(hAd-GFP), MBs(hAd-GFP) + US, or via IT with hAd-GFP. Two hours after the treatment, blood samples were collected to determine inflammatory cytokine response. At the endpoint of 4 weeks, mice were sacrificed and blood and organs were collected. Activation of the innate and adaptive immune response were evaluated in both experiments by ELISA (TNF-α, IL-6 and NAbs)