Fig. 3

Canakinumab reduces Mmp-2 expression and enzymatic activity induced by IL-1β in HK-2 cells. a Gene expression of Mmp-2 measured by Real Time-PCR in HK-2 cells treated with IL-1β or TGF-β with and without Canakinumab. Expression levels are normalized to Gapdh. Data are indicated as mean ± SD of three experiments performed in triplicate. b Gelatin zymography shows the activity of MMP-2 in the conditioned media of HK-2 cells treated for 24 h with TGF-β (10 ng/ml) or IL-1β (10 ng/ml) in the presence or absence of Canakinumab (5 μg/ml). c The histogram represents the densitometric analysis of enzymatic activity of MMP-2 as a mean ± SD of three experiments. The p value was calculated with the t-test. **p < 0.001 versus untreated control cells (CTR); ^°p < 0.05, ^°°p < 0.01 vs TGF-β untreated; ^#p < 0.05, ^##p < 0.01 vs IL-1β untreated