Fig. 8

Exosomes derived from MSCs overexpressing miR-34c attenuate radioresistance of NPC in vivo and in vitro. a Cell apoptosis in each group was analyzed by flow cytometry with APC/7-AAD staining. b Bar graph showing the rate of apoptosis in cells. c Colony formation assays assessing the radiosensitivity and the qualified number of colonies in each group. d Curves showing radioresisitance were generated and fit with an LQ model. e CCK-8 assay assessing the survival of CNE-2R after radiation. f CNE-2R cells were treated with PBS, and exosomes were injected subcutaneously into nude mice (n = 4 in each group). The mice were treated with RT at day 24. h Tumor volumes were monitored every 3 days, and g after sacrifice, tumor weights were measured. i Tumor growth rate was calculated. j Left panel: Representative images showing the IHC staining of tumor samples from different groups for β-catenin, N-cadherin, E-cadherin, Ki67, and TUNEL assays at 20-fold and 40-fold magnification. Right panel: H-score of each group (*P < 0.05; **P < 0.01; ***P < 0.001; ns no statistical significance)