Fig. 7

Exosomes derived from MSCs overexpressing miR-34c attenuate malignant behavior in NPC. a Representative images of human MSCs transfected with miR-34c or NC nucleotide sequences and corresponding electron microscopic images of exosomes. b Western blot analysis showing the presence of TSG101 and CD9 and the absence of calnexin in MSC-derived exosomes. c Particle size distribution of exosomes measured by Nanosight. d PCR analysis of miR-34c levels in exosomes. e miR-34c expression in NPCs detected by qRT-PCR after treatment with PBS or miR-34c-overexpressing (MSC-exo-miR-34c) or control (MSC-exo-NC) exosomes. f Migration ability detected by Transwell assay. g The number of cells per field in migration and invasion assays. h Photos of colonies of each group in colony formation assays and i a bar graph showing the number of colonies in different groups. j Cell proliferation in each group was measured by CCK8 assay (the results were reproduced in three independent experiments.*P < 0.05; **P < 0.01; ***P < 0.001; ns: no statistical significance)