Fig. 3

Overexpression of miR-34c promotes apoptosis and limits the radioresistance of NPC cells. a Western blot analysis of the apoptosis markers Bcl-2 and bax in CNE-2 and 5–8F cells. b Bar graph showing the ratio of Bcl-2/bax in each group. c The effect of miR-34c on cell apoptosis was analyzed by flow cytometry with APC/7-AAD staining. d The proportion of apoptotic cells. e Colony formation assays assessing the radiosensitivity of each group and the qualified number of colonies in each group. f Curves showing radio resistance were generated and fit with an LQ model. g The effects of miR-34c on the survival of CNE-2R after radiation was determined by CCK-8 assay (the results were reproduced in three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001; ns: no statistical significance)