Fig. 3

Immunolabeling of Cx43 in BAs derived from the sham and SAH groups. a Basal views of the brain in sham-operated and SAH animal. Blood was not observed in the sham group while blood are diffused in the subarachnoid space and around Willis’ circle in the SAH group; b the optic densities of Cx43 were normalized to that of sham rat at the corresponding time periods, SAH leads to increasing Cx43 expression in BAs. The upper photographs are from a representative immunoblot and quantitative graphs are shown below. Data represent the mean ± SEM, *P<0.05,**P<0.01, v.s. sham group at the corresponding time point, n = 8. The uncropped blots are presented in Additional file 1: Fig. S4. c Localization for DAPI and Cx43 in rat subjected to surgery. Tissues were taken on day 7 after surgery. White arrow: endothelium cell (EC); red arrow: smooth muscle cell (SMC); white line: internal elastic lamina. Scale bar = 15 μm. Images of day 1, 3, 5 and 14 are presented in Additional file 1: Fig. S7