Fig. 5

Venn and cluster results of IBT quantification between AIH and normal mice. a Volcano plot of differentially expressed proteins between the two groups (AIH vs. Normal). Each point represents a detected protein. The red dots indicate the up-regulated proteins, the green dots indicate the down-regulated proteins, and the gray dots indicate the non-significant differentially expressed proteins. This plot is a volcano plot of log2 fold-change (x-axis) versus − log10 Q-value (y-axis, representing the probability that the protein is differentially expressed). For the differentially expressed protein in a single test, a Q-value of < 0.05 and fold change of > 1.5 are set as the significant thresholds for differential expression. When the experiment was repeated, differentially expressed proteins were defined based on a 1.5-fold change (mean value of all comparison groups) and P-value (t-test of all comparison groups) of less than 0.05. b The number of significantly up-regulated proteins (148) and down-regulated proteins (28) in the AIH and normal groups are shown in the histogram. c Hierarchical cluster analysis results of DEPs between the AIH group and normal group. The blue color represents the down-regulated DEPs, the red color represents the up-regulated DEPs, and the white color represents the DEPs with no detectable expression change. The cluster analysis was conducted based on Euclidean distance and a hierarchical algorithm